Phospho-SirT1 (Ser27) Antibody
货号：
2327S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 2327S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | This antibody detects endogenous levels of SirT1 protein only when phosphoryated on serine 27. The antibody does not cross-react with other sirtuin proteins. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARγ (7) and the PPARγ coactivator-1α (PGC-1α) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, since it is phosphorylated on Ser27 and Ser47 in vivo, however, the function of these phosphorylation sites has not yet been determined (9). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
参考图片Western blot analysis of 293 and HCT15 cell lysates treated or untreated with lambda phosphatase using Phospho-SirT1 (Ser27) Antibody #2327 (upper) or SirT1 Antibody #2310 (lower). 使用Phospho-SirT1 (Ser27) Antibody #2327 (上图)或SirT1 Antibody #2310 (下图)，免疫印迹(Western blot)分析293和HCT15细胞中Phospho-SirT1 (Ser27)和SirT1的蛋白水平。 |
