Phospho-p130 Cas (Tyr165) Antibody
货号：
4015S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 4015S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-p130 Cas (Tyr165) Antibody detects endogenous levels of p130 Cas only when phosphorylated at tyrosine 165. The antibody may cross-react with other phosphorylated tyrosines in the substrate domain of p130 Cas. The antibody may cross-react with phosphorylated PDGFR. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | p130 Cas (Crk-associated substrate) is a docking protein containing multiple protein-protein interaction domains. The amino-terminal SH3 domain may function as a molecular switch regulating CAS tyrosine phosphorylation, as it interacts with focal adhesion kinase (FAK) (1) and the FAK-related kinase PYK2 (2), as well as with the tyrosine phosphatases PTP-1B (3) and PTP-PEST (4). The carboxy-terminal Src binding domain (SBD) contains a proline-rich motif that mediates interaction with the SH3 domains of Src-family kinases (SFKs) and a tyrosine phosphorylation site (Tyr668 and/or Tyr670) that can promote interaction with the SH2 domain of SFKs (5). The p130 Cas central substrate domain, the major region of tyrosine phosphorylation, is characterized by 15 tyrosines present in Tyr-X-X-Pro (YXXP) motifs, including Tyr165, 249 and 410. When phosphorylated, most YXXP motifs are able to serve as docking sites for proteins with SH2 or PTB domains including adaptors, C-Crk, Nck and inositol 5-phosphatase 2 (SHIP2) (6). The tyrosine phosphorylation of p130 Cas has been implicated as a key signaling step in integrin control of normal cellular behaviors including motility, proliferation and survival. Aberrant CAS tyrosine phosphorylation may contribute to cell transformation by certain oncoproteins. |
| 存放说明 | -20C |
| 计算分子量 | 130 |
参考图片Western blot analysis of extracts from NIH-3T3 cells, untreated or calf intestinal phosphotase (CIP)-treated, using Phospho-p130 Cas (Tyr165) Antibody (upper) or p130 Cas antibody (lower).未处理或经过牛小肠碱性磷酸酶（CIP）处理的NIH/3T3细胞提取物，使用Phospho-p130 Cas (Tyr165) 抗体（上）或p130 Cas抗体（下）进行western blot分析。 |
