| 货号 | 9595S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | MSK1, a mitogen and stress activated protein kinase, is activated by Erk as well as p38 MAPK in response to growth factors and cellular stress, respectively (1). MSK1 resembles RSK because it has two kinase domains connected by a regulatory linker region (2). Phosphorylation of RSK1 at Ser364 and Ser381 is critical for RSK1 activity (3). These sites are analogous to Ser360 and Ser376 of MSK1, which may be important for MSK1 activity as well. |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Western blot analysis of extracts from 293 cells, transfected with wt MSK1 or mutant MSK1, untreated or TPA-treated (200 nM), using Phospho-MSK1 (Thr581) Antibody.Western blot方法检测细胞提取物：转染MSK1、突变体MSK1的293细胞和未处理、TPA处理的(200 nM)HeLa细胞。使用的抗体是Phospho-MSK1 (Thr581) Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Phospho-MSK1 (Thr581) Antibody.免疫组织化学方法检测石蜡包埋的人乳腺癌组织，显示了细胞核位点，使用的抗体为Phospho-MSK1 (Thr581) Antibody。 | |
Western blot analysis of extracts from 293 cells, untreated, UV-treated or calyculin A-treated, using Phospho-MSK1 (Thr581) Antibody.Western blot方法检测细胞提取物：未处理、紫外照射和calyculin A处理的293细胞。使用的抗体是Phospho-MSK1 (Thr581) Antibody。 | |
Western blot analysis of extracts from 293 cells untreated or treated with UV and HeLa cells untreated or treated with calyculin A, using Phospho-MSK1 (Thr581) Antibody.Western blot方法检测细胞提取物：未处理、紫外照射293细胞和未经处理、calyculin A处理的HeLa细胞。使用的抗体是Phospho-MSK1 (Thr581) Antibody。 |
