| 货号 | 9714S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | Phospho-Histone H3 (Thr3) Antibody detects endogenous levels of histone H3 protein only when phosphorylated at Thr3. The antibody does not cross-react with other phosphorylated histones or with acetylated histones. |
| 使用方法 | WB(1:1000) IHC-P (1:50) |
| 供应商 | CST |
| 背景 | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11). |
| 存放说明 | -20C |
| 计算分子量 | 17 |
Western blot analysis of extracts from NIH/3T3 and HeLa cells, untreated or calyculin A and 20% FCS treated, using Phospho-Histone H3 (Thr3) Antibody (upper) or Histone H3 Antibody #9715 (lower). 使用Phospho-Histone H3 (Thr3) Antibody (upper) or Histone H3 Antibody #9715 (lower)，免疫印迹(Western blot)分析NIH/3T3和HeLa细胞，细胞分为untreated或calyculin A和20% FCS treated。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing nuclear localization, using Phospho-Histone H3 (Thr3) Antibody. 使用Phospho-Histone H3 (Thr3) Antibody，免疫组化分析人源结肠癌组织石蜡切片，结果显示细胞核定位。 | |
Immunohistochemical analysis of paraffin-embedded human tonsil untreated (left) or lambda phosphatase-treated (right), using Phospho-Histone H3 (Thr3) Antibody. 使用Phospho-Histone H3 (Thr3) Antibody，免疫组化分析untreated (左图)或lambda phosphatase-treated (右图)的人源扁桃体组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded MCF7 cells untreated (left) or nocodazole-treated (right), using Phospho-Histone H3 (Thr3) Antibody. 使用Phospho-Histone H3 (Thr3) Antibody，免疫组化分析untreated (左图)或lambda nocodazole-treated (右图)的MCF7细胞石蜡切片。 |
