| 货号 | 3815S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | Phospho-HDAC3 (Ser424) Antibody detects endogenous levels of HDAC3 protein only when phosphorylated on Ser424. The antibody does not cross-react with other HDAC proteins. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:200) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 49 |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or λ-phosphatase-treated (right), using Phospho-HDAC3 (Ser424) Antibody (green). Actin filaments were labeled using DY-554 phalloidin (red). 使用Phospho-HDAC3 (Ser424) Antibody (绿色)，共聚焦免疫荧光分析HeLa细胞，细胞分为untreated (左图)或λ-phosphatase-treated (右图)。DY-554 phalloidin标记微丝蛋白(红色)。 | |
Western blot analysis of extracts from HeLa, C6 and C2C12 cells, untreated or λ-phosphatase-treated, using Phospho-HDAC3 (Ser424) Antibody (upper) or HDAC3 Antibody #2632 (lower). 使用Phospho-HDAC3 (Ser424) Antibody (上图)或HDAC3 Antibody #2632 (下图)，免疫印迹(Western blot)分析HeLa、C6和C2C12细胞中Phospho-HDAC3 (Ser424)和HDAC3的蛋白水平。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-HDAC3 (Ser424) Antibody. 使用Phospho-HDAC3 (Ser424) Antibody，免疫组化分析人源肺癌组织石蜡切片。 |
