| 货号 | 5399S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Phospho-eIF4B (Ser406) Antibody detects endogenous levels of eIF4B protein only when phosphorylated at Ser406. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity (1). Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation (2-5). Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002 (6). Recently Ser406 was identified as a novel phosphorylation site regulated by mitogens (7), and the phosphorylation of this site is dependent on the MEK and mTOR activity (7). This phosphorylation is shown to be essential for the translational activity of eIF4B (7). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Western blot analysis of extracts from NIH/3T3 cells, untreated or λ phosphatase treated, using Phospho-eIF4B (Ser406) Antibody (upper) or eIF4B Antibody #3592 (lower). 使用Phospho-eIF4B (Ser406) Antibody (上图)和eIF4B Antibody #3592 (下图)，免疫印迹（Western Blot）分析在NIH/3T3细胞系中磷酸化eIF4B (Ser406)和eIF4B蛋白水平，细胞分为对照组和λ磷酸酶处理组。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (upper) or λ phosphatase treated (lower), using Phospho-eIF4B (Ser406) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). 使用Phospho-eIF4B (Ser406) Antibody（绿色）标记，共聚焦免疫荧光观察磷酸化eIF4B (Ser406)蛋白在HeLa细胞中定位，细胞分为对照组和λ磷酸酶处理组。DY-554 phalloidin (红色)标记微丝蛋白。蓝色伪彩= AQ5® #4084 (DNA荧光染料)。 |
