| 货号 | 4404S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 使用方法 | WB(1:1000) IHC-P (1:200) |
| 供应商 | CST |
| 背景 | The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for c-Cbl, an adaptor protein that leads to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provides a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10). |
| 存放说明 | -20C |
| 计算分子量 | 175 |
Western blot analysis of various cell lysates containing activated EGFR, ErbB2, ErbB3 and ErbB4 proteins, using Phospho-EGF Receptor (Tyr1148) Antibody (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower).用抗磷酸化EGFR (Tyr1148)的抗体（上）或抗磷酸化酪氨酸(P-Tyr-100)的抗体#9411（下）对含有活化的EGFR, ErbB2, ErbB3 和 ErbB4蛋白的各种细胞裂解物进行免疫印迹检测。 | |
Western blot analysis of extracts from A431 cells, untreated or EGF-stimulated, using Phospho-EGF Receptor (Tyr1148) Antibody (upper) or EGF Receptor Antibody #2232 (lower).用抗磷酸化EGFR (Tyr1148)的抗体（上）或抗EGFR的抗体#2232（下）对经过或未经EGF处理的A431细胞进行免疫印迹检测。 | |
Immunohistochemical staining of phosphorylated EGF Receptor in paraffin-embedded human breast carcinoma, showing membrane localization using Phospho-EGF Receptor (Tyr1148) Antibody.用抗磷酸化EGFR (Tyr1148)的抗体对石蜡包埋的人乳腺癌组织中磷酸化的EGFR进行免疫组化检测，图中显示胞膜阳性。 | |
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 cells, untreated (left), EGF-treated (middle) or EGF and Tarceva®- treated (right), using Phospho-EGF Receptor (Tyr1148) Antibody. 用抗磷酸化EGFR (Tyr1148)的抗体对经过（中）、未经（左）EGF处理或经过EGF和Tarceva®同时处理（右）的石蜡包埋的MDA-MB-468细胞进行免疫组化检测。 |
