Phospho-Ack1 (Tyr284) Antibody
货号：
3138S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3138S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Ack1 (Tyr284) Antibody detects transfected levels of Ack1 only when phosphorylated at Tyr284. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Ack1 and Ack2 (activated cdc42-associated kinase 1 and 2) are non-receptor tyrosine kinases that consist of a tyrosine kinase core, an SH3 domain, a cdc42/Rac-binding (CRIB) domain, a Ralt homology region and a proline-rich region (1,2). Ack1 and 2 are the only two tyrosine kinases known to interact with cdc42. Both Acks are activated by growth factors including EGF and PDGF, as well as by activated integrins through cell adhesion, and may serve to link receptor tyrosine kinase or G protein-coupled receptor signaling with cdc42. Acks may regulate cell growth, morphology and motility (3,4). Recent findings indicate that Ack1 may play a role in prostate tumorigenesis, making it a potential drug target for this type of cancer (5). |
| 存放说明 | -20C |
| 计算分子量 | 135 |
参考图片Western blot analysis of extracts from COS cells, untransfected or overexpressing Myc-tagged Ack1 protein, using Phospho-Ack1 (Tyr284) Antibody (upper and middle) and Myc-Tag (71D10) Rabbit mAb #2278 (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing. (The lysates of COS cells overexpressing Myc-tagged Ack1 were kindly provided by Dr. Wannian Yang, Weis Center for Research, Geisinger Clinic.)使用Phospho-Ack1 (Tyr284) Antibody (上图和中图)和Myc-tag (71D10) Antibody #2278 (下图)，免疫印迹（Western Blot）分析在COS细胞系中未转染、转染的Myc标记的人源Ack1表达质粒。中图在抗体检测之前使用calf intestinal phosphatase (CIP)处理。（转染Myc标记的人源Ack1表达质粒的COS细胞裂解液是由Dr. Wannian Yang, Weis Center for Research, Geisinger Clinic友好提供。） |
