Phospho-5-Lipoxygenase (Ser663) Antibody
货号：
3749S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3749S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-5-Lipoxygenase (Ser663) Antibody detects overexpressed phospho-5-lipoxygenase protein only when phosphorylated at Ser663. |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | 5-Lipoxygenase (5-LO, ALOX5) is an important catalytic enzyme responsible for the biosynthesis of leukotriene LTA4 from arachidonic acid (1,2). Leukotriene synthesis also requires 5-lipoxygenase-activating protein (FLAP, ALOX5AP), a nuclear membrane-bound protein that binds arachidonic acid and is thought to activate 5-LO. A number of related leukotrienes (i.e. B4, C4, D4) are derived from LTA4 and together these lipid mediators function in immune reaction regulation. 5-LO is primarily expressed in polymorphonuclear leukocytes, peripheral blood monocytes, macrophages, and mast cells (1,3). Overexpression of 5-LO protein is seen in certain cancer cells and is associated with poor diagnosis (1,4). Depending upon the cell type, 5-LO is localized to either the cytosol or the nucleus of quiescent cells (5). Following stimulation, 5-LO translocates to the nucleus and associates with FLAP to catalyze LTA4 synthesis (2,3). Phosphorylation of specific residues can regulate 5-LO enzymatic activity. Phosphorylation of 5-LO at Ser523 by PKA family kinases inhibits oxygenase activity (6,7) while MAPKAP2 and ERK family kinase phosphorylation at Ser271 and Ser663 stimulates 5-LO enzymatic activity in vivo (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 78, 80 |
参考图片Western blot analysis of extracts from COS cells, untransfected, or transfected with either 5-LO wild type or GFP 5-LO (Ser663Ala), using Phospho-5-Lipoxygenase (Ser663) Antibody (upper) and 5-Lipoxygenase (C49G1) Rabbit mAb #3289 (lower). (We are thankful to Dr. Flamand from University of Michigan for providing the overexpression constructs and for his help in characterizing this antibody).Western免疫印迹。用Phospho-5-Lipoxygenase (Ser663) Antibody (上图)和5-Lipoxygenase (49G1) Rabbit mAb #3289(下图)研究未转染和转染了野生型5-LO 或 GFP 5-LO (Ser663Ala)的COS细胞的细胞提取液。(感谢University of Michigan的Flamand博士提供了过表达质粒以及帮助纯化抗体) |
Western blot analysis of extracts from COS cells, transfected with 5-LO wild type or GFP 5-LO (Ser663Ala), using Phospho-5-Lipoxygenase (Ser663) Antibody. The phospho-specificity of the antibody was verified by preincubating the antibody with no peptide (left), with 5-LO (Ser663) non-phosphopeptide (middle) or 5-LO (Ser663) phosphopeptide (right) prior to incubating the membrane.Western免疫印迹。用Phospho-5-Lipoxygenase (Ser663) Antibody研究转染了野生型5-LO 或GFP 5-LO (Ser663Ala)的COS细胞的细胞提取液。磷酸化特异性抗体是通过在与膜孵育之前先与没有多肽的(左图)，未磷酸化5-LO (Ser663)多肽(中间图)或5-LO (Ser663)磷酸化多肽(右图)预培养。 |
