| 货号 | 4381S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W/E-P |
| 目标/特异性 | Phospho-(Ser/Thr) PKD Substrates Antibody detects peptides and proteins containing a phospho-Ser/Thr residue with arginine at the -3 position and leucine at the -5 position, prefering proline at the -1 position within the motif. The antibody may cross-react with some peptides and proteins containing an arginine residue at -3 position and a hydrophobic or basic residue at the -5 position. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) E-P (1:1000) |
| 供应商 | CST |
| 背景 | Protein kinase D1 (PKD1), also called PKCμ, is one member of the protein kinase D family. PKD members are serine/threonine protein kinases implicated in very diverse cellular functions including cell growth and survival, Golgi organization and immune response (1). The information about the natural substrates of PKD is limited (2,3). The enzyme recognizes and phosphorylates the following consensus sequence: LXRXXS/T. Phospho-(Ser/Thr) PKD Substrate Antibody recognizes phosphorylated PKD substrates at their consensus motif, providing a powerful new tool for PKD target discovery and characterization as well as HTS drug screening for potential kinase regulators. |
| 存放说明 | -20C |
Western blot analysis of extracts from HeLa cells, untreated or PMA-treated (100 nM), using PKD Substrates Antibody. Arrows point to PMA-inducible bands. (Provided by Dr. Alex Toker, Beth Israel Deaconess Medical Center, Harvard Medical School). 对PC12细胞，未处理或100nM PMA处理，使用PKD Substrates Antibody进行Western blot分析。箭头指示PMA诱导的条带。（Dr. Alex Toker, Beth Israel Deaconess Medical Center, Harvard Medical School提供） | |
Phospho-(Ser/Thr) PKD Substrates Antibody DELFIA® Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.) (DELFIA® is a registered trademark of PerkinElmer, Inc.) Phospho-(Ser/Thr) PKD Substrates Antibody DELFIA®分析：磷酸化和非磷酸化的信噪比。（T*和S*代表磷酸化的苏氨酸和丝氨酸）（DELFIA®是PerkinElmer公司的注册商标） | |
Western blot analysis of extracts from HeLa cells transfected with pcDNA or with activated PKD (S744E/S748E), using PKD Substrates Antibody (Arrow points to active PKD as well as inducible bands). 对HeLa细胞，转染pcDNA或激活的pKD（S744E/S748E），使用PKD Substrates Antibody进行Western blot分析（箭头指示激活的PKD）。 | |
Western blot analysis of extracts from COS-7 cells, untreated or treated with TPA (#4174, 200 nM, 20 min), using the Phospho-(Ser/Thr) PKD Substrates Antibody. 对COS-7细胞抽提物，未处理或用TPA(#4174, 200 nM, 20 min)处理，使用Phospho-(Ser/Thr) PKD Substrates Antibody进行免疫印迹分析 |
