| 货号 | 9631S |
| 反应种属 | Human/Mouse/Rat/All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/E-P |
| 目标/特异性 | Phospho-(Ser/Thr) Phe Antibody detects phospho-serine or threonine in the context of tyrosine, tryptophan or phenylalanine at the -1 position or phenylalanine at the +1 position. The antibody does not cross-react with the nonphosphorylated form of these motifs, nor does it cross-react with other phospho-serine/threonine-containing proteins and peptides. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) IP (1:100) E-P (1:1000) |
| 供应商 | CST |
| 背景 | A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases (1), but also those that are recognized by phosphorylation-dependent binding proteins such as 14-3-3 (2). These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. CST has pioneered the development of phospho-motif specific antibodies, which are invaluable tools for probing the complexity of phospho-regulatory pathways. |
| 存放说明 | -20C |
Phospho-(Ser/Thr) Phe Antibody ELISAs: Signal to noise ratio of phospho- versus nonphospho-peptides in which the phosphorylation site is adjacent to phenylalanine, tyrosine or tryptophan. (T* and S* denote phosphorylated threonine and serine.) Phospho-(Ser/Thr) Phe Antibody ELISA分析：磷酸化和非磷酸化多肽的信噪比，磷酸化位点毗邻苯丙氨酸、酪氨酸和色氨酸。（T*和S*代表磷酸化的苏氨酸和丝氨酸） | |
Western blot analysis of extractss from A431 cells, untreated or calyculin A-treated, using Phospho-(Ser/Thr) Phe Antibody. 对未处理或calyculin A处理的A431细胞抽提液使用Phospho-(Ser/Thr) Phe Antibody进行Western blot分析。 |
