| 货号 | 2261S |
| 反应种属 | All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/E-P |
| 目标/特异性 | Phospho-(Ser) PKC Substrate Antibody detects endogenous levels of many cellular proteins only when phosphorylated at serine residues surrounded by Arg or Lys at the -2 and +2 positions and a hydrophobic residue at the +1 position. The antibody does not cross-react with nonphosphorylated serine residues, with phospho-threonine in the same motif, or with phospho-serine in other motifs. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) IP (1:25) E-P (1:500) |
| 供应商 | CST |
| 背景 | Although protein kinase C (PKC) family members are involved in a number of signal transduction processes including secretion, gene expression, proliferation, and muscle contraction (1,2), many of their substrates remain unidentified. Isozymes of PKC are subdivided into conventional PKCs (cPKC), novel PKCs (nPKC), and atypical PKCs (aPKC). PKCα, βI, βII, and γ isoforms belong to the cPKC group (1). When activated, cPKC isozymes phosphorylate substrates containing Ser or Thr, with Arg or Lys at the -3, -2, and +2 positions, and a hydrophobic amino acid at position +1 (1-3). To identify potential PKC substrates, Cell Signaling Technology has developed antibodies that recognize this motif. Phospho-(Ser) PKC Substrate (D13C5/D47H10/D50E3) Rabbit mAb is specific to cPKC substrates containing phospho-Ser. |
| 存放说明 | -20C |
Western blot analysis of extracts from Jurkat cells, untreated or treated with PMA (50 ng/ml) and ionomycin (1 µM for 20 minutes prior to lysis) and subjected to 2-D electrophoresis, using Phospho-(Ser) PKC Substrate Antibody. 对未处理或50ng/ml PMA和1uM ionomycin裂解前处理20分钟的Jurkat细胞抽提液进行二维电泳，使用Phospho-(Ser) PKC Substrate Antibody进行Western blot分析。 | |
Western blot analysis of extracts from WEHI 231 cells and Jurkat cells, untreated or TPA-treated, using Phospho-(Ser) PKC Substrate Antibody. 对未处理或TPA处理的WEHI 231细胞和Jurkat细胞抽提液使用Phospho-(Ser) PKC Substrate Antibody进行Western blot分析。 | |
Phospho-(Ser) PKC Substrate Antibody ELISA assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (S* denotes phosphorylated serine.) Phospho-(Ser) PKC Substrate Antibody ELISA分析：磷酸化和非磷酸化的信噪比。（S* 代表磷酸化的丝氨酸） |
