| 货号 | 2981S |
| 反应种属 | Human/Mouse/All |
| 来源宿主 | Rabbit |
| 应用 | W/IP/E-P |
| 目标/特异性 | Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody detects endogenous levels of proteins containing the Arg-X-Tyr/Phe-X-pSer motif. This antibody does not cross-react with nonphosphorylated serine or phospho-threonine proteins with the same motif or other phospho-serine/threonine-containing proteins and peptides without this motif. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) IP (1:50) E-P (1:1000) |
| 供应商 | CST |
| 背景 | The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control (1-5). Binding of 14-3-3 is mediated through phospho-serine-containing proteins (6). Two different phospho-serine containing motifs are found using a degenerate phospho-serine-oriented peptide library technique, RSXS*XP and RXY/FXS*XP (6). Motif 2 (RXY/FXS*XP) is found in critical regulatory proteins including cdc25A, cdc25B, PKCgamma, IRS-1 and BCR (6). Although Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody binds 14-3-3 binding motif 2 with no requirement for proline in the +2 position, it provides a powerful tool for the discovery and characterization of potential 14-3-3 binding motif 2-containing proteins or other proteins with the RXY/FXS* motif. |
| 存放说明 | -20C |
Western blot analysis of extracts from A431 cells, untreated or calyculin A treated, using Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody. 对未处理或calyculin A处理的A431细胞抽提液使用Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody进行Western blot分析。 |
