Nitro-Tyrosine Antibody
货号：
9691S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 9691S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Nitro-Tyrosine Antibody detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples. The antibody does not cross-react with unmodified tyrosine or with phospho-tyrosine. (U.S. Patent Nos.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.) |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Nitric oxide (NO) is implicated in carcinogenesis (1), chronic infection, inflammation (2) and neurodegeneration (3). High levels of both superoxide and nitric oxide in tissues interact to form peroxynitrite, a potent oxidant that can modify Tyr residues in proteins to form 3-nitro-tyrosine (4). Tyrosine nitration of mitochondrial manganese superoxide dismutase results in loss of enzymatic activity (4). The nitration of p53 at Tyr residues abolishes its capacity for binding to its DNA consensus sequence (5). |
| 存放说明 | -20C |
参考图片Western blot analysis of extracts from various cell lines, untreated or treated with peroxynitrite, degraded peroxynitrite or pervanadate, using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (upper) or Nitro-Tyrosine Antibody (lower). 对多个细胞系抽提物，未处理或过氧亚硝基阴离子处理适当时间，降解过氧亚硝基阴离子或过钒酸钠，使用Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411（上图）或Nitro-Tyrosine Antibody（下图）进行Western blot分析。 |
