| 货号 | 9398S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Mono-Methyl-Histone H3 (Lys79) Antibody recognizes endogenous levels of histone H3 protein only when mono-methylated at Lys79. The antibody does not cross-react with non-methylated, di-methylated, or tri-methylated histone H3 Lys79. In addition, the antibody does not cross-react with histone H3 mono-methylated at Lys4, Lys9, Lys27, or Lys36. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker (9). |
| 存放说明 | -20C |
| 计算分子量 | 17 |
Western blot analysis of extracts from various cell lines using Mono-Methyl-Histone H3 (Lys79) Antibody. 使用Mono-Methyl-Histone H3 (Lys79) Antibody，免疫印迹(Western blot)分析不同细胞中Mono-Methyl-Histone H3 (Lys79)蛋白水平。 | |
Mono-Methyl-Histone H3 (Lys79) Antibody specificity was determined by peptide ELISA. The graph depicts the binding of the antibody to pre-coated mono-methyl-histone H3 (Lys79) peptide in the presence of increasing concentrations of various competitor peptides. As shown, only the mono-methyl-histone H3 (Lys79) peptide competed away binding of the antibody. |
