Acetyl-NF-κB p65 (Lys310) Antibody
货号：
3045S 基本售价：
3980.0 元 规格：
-
产品信息
概述| 货号 | 3045S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Acetyl-NF-κB p65 (Lys310) Antibody detects transfected levels of NF-κB only when acetylated at Lys310. |
| 使用方法 | WB(1:1000)
IP (1:50) |
性能| 供应商 | CST |
| 背景 | Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11). |
| 存放说明 | -20C |
| 计算分子量 | 65 |
参考图片Western blot analysis of extracts from HeLa cells, mock transfected or transfected with NF-κB or NF-κB plus CBP, using Acetyl-NF-κB (Lys310) Antibody. Western blots were competed with acetylated peptide (middle) or non-acetylated peptide (right).Western免疫印迹。用 Acetyl-NF-κB (Lys310) Antibody研究对照转染和转染了NF-κB 或 NF-κB 加上CBP的 HeLa 细胞的细胞提取液。Western免疫印迹通过与乙酰基化得多肽(中间图) 竞争或未乙酰基化的多肽 (右图)竞争。 |
