| 货号 | 49281S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-MFF (Ser146) Antibody recognizes endogenous levels of MFF protein only when phosphorylated at Ser146. This antibody has been shown to react with phosphorylated Ser146 of human MFF isoforms 2, 4, and 5 but not phosphorylated Ser172 of human MFF isoform 1 (7). This antibody cross-reacts with a 140kDa protein of unknown identity. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Mitochondrial fission factor (MFF) is a tail-anchored protein that resides within the outer mitochondrial membrane and is part of the mitochondrial fission complex. MFF participates in mitochondrial fission by serving as one of multiple receptors for the GTPase dynamin-related protein 1 (Drp1) (1-4). Research studies have also shown that MFF is a peroxisomal membrane protein and participates in peroxisome fission by serving as a receptor for another GTPase, dynamin-like protein 1 (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 25, 27 |
| 参考文献 | 1 . Liu, R. and Chan, D.C. (2015) Mol Biol Cell 26, 4466-77. 2 . Shen, Q. et al. (2014) Mol Biol Cell 25, 145-59. 3 . Losón, O.C. et al. (2013) Mol Biol Cell 24, 659-67. 4 . Otera, H. et al. (2010) J Cell Biol 191, 1141-58. 5 . Itoyama, A. et al. (2013) Biol Open 2, 998-1006. 6 . Gandre-Babbe, S. and van der Bliek, A.M. (2008) Mol Biol Cell 19, 2402-12. 7 . Toyama, E.Q. et al. (2016) Science 351, 275-81. 8 . Ducommun, S. et al. (2015) Cell Signal 27, 978-88. |
Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with CCCP (100uM, 2hr; +), using Phospho-MFF (Ser146) Antibody. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after protein transfer. | |
Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with CCCP (100μM, 2hr; +), using phospho-MFF (Ser146) Antibody (upper) or total MFF antibody (lower). |
