| 货号 | 2735S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | XRCC1 Antibody detects endogenous levels of total XRCC1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The X-ray repair cross complementing protein 1 (XRCC1) is a DNA repair protein important in both single strand break repair and base excision repair following damage from ionizing radiation and alkylating agents (1). XRCC1 acts as a scaffold protein to coordinate DNA abasic site repair through interaction with several other repair proteins (2). At least eight XRCC1 protein partners have been identified, including the polynucleotide kinase PNK (3), DNA ligase III (4,5), poly (ADP-ribose) polymerase (6), and PCNA (7). Mutations and polymorphisms in the XRCC1 gene serve as diagnostic markers and are associated with elevated risk of various forms of cancers (8).X射线修复交叉互补蛋白1(XRCC1)是一个DNA修复蛋白，它在伴随离子辐射和烷化剂而至的单链断裂修复和碱基清除修复中都发挥重要作用(1)。XRCC1能够充当支架蛋白并通过与其它几个修复蛋白相互作用来协调DNA脱碱基位点修复(2)。至少八个XRCC1蛋白伴侣已经被鉴定，包括多核苷酸激酶PNK(3)，DNA连接酶III (4,5)，多(ADP-核糖)聚合酶(6)和PCNA (7)。XRCC1基因的突变和多态性可充当诊断标志物并和多种不同类型癌症的高风险相关(8)。 |
| 存放说明 | -20C |
| 计算分子量 | 82 |
| 参考文献 | 1 . Brem, R. and Hall, J. (2005) Nucleic Acids Res. 33, 2512-2520. 2 . Vidal, A.E. et al. (2001) EMBO J 20, 6530-9. 3 . Whitehouse, C.J. et al. (2001) Cell 104, 107-117. 4 . Caldecott, K.W. et al. (1994) Mol. Cell. Biol. 14, 68-76. 5 . Nash, R.A. et al. (1997) Biochemistry 36, 5207-5211. 6 . Masson, M. et al. (1998) Mol. Cell. Biol. 18, 3563-3571. 7 . Fan, J. et al. (2004) Nucleic Acids Res. 32, 2193-2201. 8 . Hu, Z. et al. (2005) Cancer Epidemiol. Biomarkers Prev. 14, 1810-1818. |
Western blot analysis of extracts from Jurkat and K562 cells using XRCC1 Antibody.Western blot方法检测Jurkat和k562细胞提取物，使用的抗体为XRCC1 Antibody。 | |
Confocal immunofluorescent analysis of HeLa cells using XRCC1 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).激光共聚焦免疫荧光方法检测Hela细胞，使用的抗体为XRCC1 Antibody，呈绿色。肌动蛋白纤维由Alexa Fluor® 555 鬼笔环肽标记，呈红色。 |
