| 货号 | 8749S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | WFS1 Antibody recognizes endogenous levels of total WFS1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Wolfram syndrome protein (WFS1) is an 890 amino acid protein that contains a cytoplasmic N-terminal domain, followed by nine-transmembrane domains and a luminal C-terminal domain. WFS1 is predominantly localized to the endoplasmic reticulum (ER) (1) and its expression is induced in response to ER stress, partially through transcriptional activation (2,3). Research studies have shown that mutations in the WFS1 gene lead to Wolfram syndrome, an autosomal recessive neurodegenerative disorder defined by young-onset, non-immune, insulin-dependent diabetes mellitus and progressive optic atrophy (4). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Takeda, K. et al. (2001) Hum Mol Genet 10, 477-84. 2 . Yamaguchi, S. et al. (2004) Biochem Biophys Res Commun 325, 250-6. 3 . Ueda, K. et al. (2005) Eur J Endocrinol 153, 167-76. 4 . Inoue, H. et al. (1998) Nat Genet 20, 143-8. |
Western blot analysis of extracts from various cell lines using WFS1 Antibody. Western blot方法检测多个细胞系提取物，使用的抗体为WFS1 Antibody. | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® WFS1 siRNA I #7044 (+), using WFS1 Antibody (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower). Western blot方法检测HeLa 细胞提取物，使用的抗体为WFS1 Antibody (上图)和β-Actin (13E5) Rabbit mAb #4970 (下图). 该细胞用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) 或SignalSilence® WFS1 siRNA I #7044 (+)转染。 |
