| 货号 | 2792S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | Tyrosine Hydroxylase Antibody detects endogenous levels of total tyrosine hydroxylase. |
| 使用方法 | WB(1:1000) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms (1,2). This enzyme is required for embryonic development since TH knockout mice die before or at birth (3). Levels of transcription, translation and posttranslational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31 and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH (4-6). Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels (7-9). |
| 存放说明 | -20C |
| 计算分子量 | 55-60 |
| 参考文献 | 1 . Kumer, S.C. and Vrana, K.E. (1996) J Neurochem 67, 443-62. 2 . Bodeau-Péan, S. et al. (1999) J Biol Chem 274, 3469-75. 3 . Kobayashi, K. et al. (1995) J Biol Chem 270, 27235-43. 4 . Lew, J.Y. et al. (1999) Mol Pharmacol 55, 202-9. 5 . Vié, A. et al. (1999) J Biol Chem 274, 16788-95. 6 . Lindgren, N. et al. (2000) J Neurochem 74, 2470-7. 7 . Moy, L.Y. and Tsai, L.H. (2004) J Biol Chem 279, 54487-93. 8 . Lehmann, I.T. et al. (2006) J Biol Chem 281, 17644-51. 9 . Saraf, A. et al. (2007) J Biol Chem 282, 573-80. |
Western blot analysis of extracts from SK-N-MC (human), SH-SY5Y (human) and PC12 (rat) cells as well as rat cerebrum and mouse whole brain tissue, using Tyrosine Hydroxylase Antibody. Western blot分析人SK-N-MC细胞、人SH-SY5Y细胞、大鼠PC12细胞、大鼠小脑和小鼠全脑组织，使用的抗体是Tyrosine Hydroxylase Antibody 兔多抗。 | |
Confocal immunofluorescent images of PC-12 cells, untreated (A,C) and calf intestinal phosphatase treated (B), labeled with Phospho-Tyrosine Hydroxylase (Ser40) Antibody #2791 (green, top) and Tyrosine Hydroxylase Antibody #2792 (green, bottom) compared to an isotype control (D). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析PC-12细胞，未处理（A,C）和小牛肠碱性磷酸酶处理（B），使用的抗体是 Phospho-Tyrosine Hydroxylase (Ser40) Antibody 兔多抗#2791 (绿色，顶图 ) 和Tyrosine Hydroxylase Antibody 兔多抗 #2792 (绿色，底图) ，同型对照（D）用作对照。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 |
