| 货号 | 8461S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Twinfilin-1 Antibody recognizes endogenous levels of total twinfilin-1 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Twinfilin is an actin monomer-binding protein found in all eukaryotes (1). Mammals have three isoforms. Twinfilin-1 and twinfilin-2a are expressed in most non-muscle cell types, whereas twinfilin-2b is the main isoform in adult heart and skeletal muscle (2). Twinfilins are composed of two ADF-homology domains connected by a 30 kDa linker region. All twinfilins have been shown to form a 1:1 complex with G-actin, but not F-actin (reviewed in 3). Twinfilin-1 was originally known as A6 protein tyrosine kinase and thought to be part of a novel class of protein kinases. However, the protein was renamed after further studies showed no evidence of tyrosine kinase activity (4). Twinfilin-1 helps to prevent the actin filament assembly by forming a complex with actin monomers and, in mammals, has been shown to cap the filament barbed ends. It has been suggested that this regulates cell motility (5). Suppression of twinfilin-1 has also been shown to slow lymphoma cell migration to lymph nodes (6). |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . Goode, B.L. et al. (1998) J Cell Biol 142, 723-33. 2 . Nevalainen, E.M. et al. (2009) Biochem J 417, 593-600. 3 . Palmgren, S. et al. (2002) J Cell Sci 115, 881-6. 4 . Vartiainen, M. et al. (2000) Mol Cell Biol 20, 1772-83. 5 . Helfer, E. et al. (2006) EMBO J 25, 1184-95. 6 . Meacham, C.E. et al. (2009) Nat Genet 41, 1133-7. |
Western blot analysis of extracts from various cell lines using Twinfilin-1 Antibody.使用Twinfilin-1抗体对多种细胞提取物进行western blot分析。 |
