| 货号 | 5182S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | TRIM29/ATDC Antibody detects endogenous levels of TRIM29/ATDC protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tipartite motif-containing protein 29 (TRIM29 or ATDC) was isolated as a candidate gene by its ability to complement the radiosensitivity defect of an ataxia-telangiectasia (AT) cell line (1). This putative transcription regulator belongs to the TRIM (tripartite motif) protein family that is characterized by highly conserved amino-terminal RING finger, B-box, and coiled-coil domains. TRIM29 binds and sequesters cytosolic p53, repressing expression of p53 target genes including p21 and Noxa by preventing p53 from entering the nucleus. Expression of TRIM29 inhibits p53 function and results in increased cell proliferation. (2). TRIM29 enhances tumor growth and metastasis in vivo and high TRIM29 levels are seen in most invasive pancreatic cancers. The oncogenic effect of TRIM29 appears to require β-catenin as expression of both proteins is elevated in pancreatic cancer cell lines and tissues (3). |
| 存放说明 | -20C |
| 计算分子量 | 66 |
| 参考文献 | 1 . Kapp, L.N. et al. (1992) Am J Hum Genet 51, 45-54. 2 . Yuan, Z. et al. (2010) Mol Cell Biol 30, 3004-15. 3 . Wang, L. et al. (2009) Cancer Cell 15, 207-19. |
Western blot analysis of extracts from SW480, SW620, and HT-29 cells using TRIM29/ATDC Antibody. 使用TRIM29/ATDC Antibody，免疫印迹(Western blot)分析SW480、SW620和HT-29细胞中TRIM29/ATDC的蛋白水平。 |
