| 货号 | 13689S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | TRIAD1 Antibody recognizes endogenous levels of total TRIAD1 protein. Based upon sequence alignment, this antibody is not predicted to cross-react with HHARI/ARIH1. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The E3 ubiquitin-protein ligase ARIH2 (TRIAD1) is an Ariadne subfamily ligase involved in the polyubiquitination of proteins designated for proteasomal degradation. The TRIAD1 nuclear protein contains an amino-terminal acidic region, a pair of RING fingers, two carboxyl-terminal coiled coil domains and a novel C6HC DRIL/IBR domain located between the RING fingers. Together, the paired RING fingers and DRIL/IBR domain form a highly conserved TRIAD (two RING fingers and DRIL) domain (1). Research studies suggest that TRIAD1 mediates both Lys48 and Lys63 protein polyubiquitination and acts as a negative regulator of myelopoiesis. TRIAD1 ubiquitin ligase inhibits myeloid cell proliferation by mediating protein ubiquitination through the ubiquitin-conjugating enzymes UbcH7 and UbcH13 (2,3). Experimental deletion of TRIAD1 in mice has a lethal effect, leading to death at the embryonic stage or later due to a severe, multi-organ inflammatory response. Results indicate that TRIAD1 binds IκBβ in dendritic cells and promotes the degradation of the NF-κB inhibitor (4). |
| 存放说明 | -20C |
| 计算分子量 | 58 |
| 参考文献 | 1 . van der Reijden, B.A. et al. (1999) Protein Sci 8, 1557-61. 2 . Marteijn, J.A. et al. (2005) Blood 106, 4114-23. 3 . Marteijn, J.A. et al. (2009) Leukemia 23, 1480-9. 4 . Lin, A.E. et al. (2013) Nat Immunol 14, 27-33. |
Western blot analysis of extracts from various cell lines using TRIAD1 Antibody. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human TRIAD1 (hTRIAD1-Myc/DDK; +), using TRIAD1 Antibody. | |
Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® TRIAD1 siRNA Ι #13719 (+), using TRIAD1 Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The TRIAD Antibody confirms silencing of TRIAD1 expression, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control. |
