| 货号 | 4482S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | THEMIS Antibody recognizes endogenous levels of total THEMIS protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | THEMIS is a recently identified protein found to be critical for T cell development (1-5). It contains two amino-terminal globular domains, a nuclear localization signal, and a carboxy-terminal proline-rich sequence with homology to SH3 binding domains (2,3). THEMIS is detected in both the cytoplasm and the nucleus (2). It is expressed at low levels in mature T cells and during thymocyte development, with expression peaking in CD4+CD8+ double positive cells (1-5). THEMIS is tyrosine phosphorylated downstream of TCR signaling and associates with the adaptor GRB2 and possibly other SH3 domain-containing proximal TCR signaling molecules (1-5). Mice lacking THEMIS have a defect in positive selection that results in decreased numbers of both single positive thymocytes and mature T cells (1-5). |
| 存放说明 | -20C |
| 计算分子量 | 74 |
| 参考文献 | 1 . Johnson, A.L. et al. (2009) Nat Immunol 10, 831-9. 2 . Lesourne, R. et al. (2009) Nat Immunol 10, 840-7. 3 . Fu, G. et al. (2009) Nat Immunol 10, 848-56. 4 . Kakugawa, K. et al. (2009) Mol Cell Biol 29, 5128-35. 5 . Patrick, M.S. et al. (2009) Proc Natl Acad Sci U S A 106, 16345-50. |
Western blot analysis of extracts from various cell lines and human primary T cells using THEMIS Antibody.Western免疫印迹。用THEMIS Antibody研究各种细胞系和人原代T细胞的细胞提取液。 |
