| 货号 | 3711S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | TGF-beta Antibody detects recombinant TGF-beta1, TGF-beta2, and TGF-beta3. The antibody also detects endogenous levels of the TGF-beta precursor proteins. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Transforming growth factor-β (TGF-β) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-4). TGF-β elicits signaling through three cell surface receptors: type I (RI), type II (RII) and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases (5). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain (6,7). Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex. The only known direct TGF-β signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 12, 25, 45 to 65 |
| 参考文献 | 1 . Massague, J. et al. (2000) Cell 103, 295-309. 2 . Caestecker, M.P. et al. (2000) J. Natl. Cancer Inst. 92, 1388-1402. 3 . Derynck, R. et al. (2001) Nature Genet. 29, 117-129. 4 . Miyazono, K. et al. (2000) Adv. Immunol. 75, 115-157. 5 . Derynck, R. et al. (1997) Biochim. Biophys. Acta. 1333, F105-150. 6 . López-Casillas, F. et al. (1991) Cell 67, 785-795. 7 . Wang, X.F. et al. (1991) Cell 67, 797-805. 8 . Derynck, R. et al. (1998) Cell 95, 737-40. 9 . Massague, J. et al. (2000) Nat. Rev. Mol. Cell Biol. 1, 169-178. 10 . Kingsley, D.M. (1994) Genes Dev 8, 133-46. |
Western blot analysis of extracts from HCT 116, A20, and NBT-II cells using TGF-beta Antibody. | |
Western blot analysis of recombinant human TGF-beta1, TGF-beta2, and TGF-beta3 using TGF-beta Antibody. |
