| 货号 | 4562S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | TFII-I Antibody detects endogenous levels of total TFII-I (all isoforms). |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TFII-I (also known as SPIN and BAP-135) is a mutifunctional transcription factor that facilitates basal transcriptional machinery assembly at the core promotor region, as well as the assembly of the transcriptional activator complex at upstream regulatory sites (1). Four isoforms of TFII-I (alpha, beta, gamma, and delta) form homo- or heteromeric complexes, which may perform different functions on different promoters (1). In B cells, cross-linking of BCR (B cell receptors) leads to TFII-I phosphorylation at Tyr248 by Btk (2). This phosphorylation disrupts the association of Btk and TFII-I and enhances TFII-I transcriptional activity and nuclear localization (2). In nonlymphoid cells, TFII-I is phosphorylated at Tyr248 by Src dependent kinase or JAK2 (3,4). PKG (cGMP-dependent kinase) interacts with and phosphorylates TFII-I at Ser371 and 743, which also promotes TFII-I transcription activity (5). TFII-I activity is also modulated by HDAC3 (Histone Deacetylase 3) through a physical interaction between the two proteins (6). |
| 存放说明 | -20C |
| 计算分子量 | 135, 138 |
| 参考文献 | 1 . Roy, A.L. (2001) Gene 274, 1-13. 2 . Novina, C. D. et al. (1999) Mol. Cell. Biol. 19, 5014-5024. 3 . Kim, D. and Cochran, B.H. (2001) Mol. Cell. Biol. 21, 3387-3397. 4 . Cheriyath, V. et al. (2002) J. Biol. Chem. 277, 22798-22805. 5 . Casteel, D. E. et al. (2002) J. Biol. Chem. 277, 32003-32014. 6 . Wen, Y. et al. (2003) J. Biol. Chem. 278, 1841-1847. |
Western blot analysis of extracts from HeLa, HEK293, U-937, COS, C2C12 and WEHI-231 cells, using TFII-I Antibody. 使用TFII-I Antibody，免疫印迹(Western blot)分析HeLa、HEK293、U-937、COS、C2C12 和WEHI-231细胞系中TFII-I的蛋白水平。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using TFII-I Antibody. 使用TFII-I Antibody，免疫组化分析人源肺癌组织石蜡切片。 |
