| 货号 | 2803S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Surivivin Antibody detects endogenous levels of total human survivin. The antibody does not cross-react with other types of apoptotic inhibitor proteins at physiological conditions. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy (1). Survivin binds and inhibits caspase-3, controlling the checkpoint in the G2/M-phase of the cell cycle by inhibiting apoptosis and promoting cell division (2,3). This regulatory process requires the phosphorylation of survivin at Thr34 by p34 cdc2 kinase (4). Gene targeting using a Thr34 phosphorylation-defective survivin mutant, as well as antisense survivin, have been shown to inhibit tumor growth (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 16 |
| 参考文献 | 1 . Reed, J.C. and Reed, S.I. (1999) Nature Cell Biol. 1, 199-200. 2 . Li, F. et al. (1998) Nature 396, 580-584. 3 . Li, F. et al. (1999) Nat. Cell Biol. 1, 461-466. 4 . OConnor, D.S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 13103-13107. 5 . Olie, R.A. et al. (2000) Cancer Res. 60, 2805-2809. 6 . Grossman, D. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 635-640. |
Western blot analysis of extracts from HT29 and Jurkat cells, using Survivin Antibody. Western blot 检测 HT29和Jurkat细胞提取物，使用抗体为Survivin Antibody。 | |
Immunoprecipitation of lysates from Jurkat cells using either control rabbit IgG or Survivin Antibody and then probed by Western blot, using Survivin (6E4) Mouse mAb #2802. 免疫沉淀分析Jurkat细胞裂解液，使用抗体为control rabbit IgG或Survivin Antibody。然后，Western blot分析，使用抗体是Survivin (6E4) Mouse mAb 鼠单抗#2802。 |
