| 货号 | 4817S |
| 反应种属 | Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | STEP Antibody detects endogenous levels of total STEP61 and may detect STEP46 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Striatal enriched phosphatase (STEP, also known as PTPN5), is a protein tyrosine phosphatase expressed in dopaminoceptive neurons of the central nervous system (1). Alternative splicing produces the cytosolic STEP46 and the membrane-associated STEP61 isoforms of STEP. Dopamine activates D1 receptors and PKA, which in turn phosphorylate both isoforms of STEP. Phosphorylation of STEP61 occurs at Ser160 and Ser221, while STEP46 is phosphorylated at Ser49 (equivalent to Ser221 of STEP61) (2). NMDA-mediated activation of STEP is an important mechanism for regulation of ERK activity in neurons (3). Furthermore, STEP is involved in the regulation of both NMDAR and AMPAR trafficking (4,5). Due to its importance in cognitive function, STEP may play a role in Alzheimer disease (1). |
| 存放说明 | -20C |
| 计算分子量 | 61 |
| 参考文献 | 1 . Braithwaite, S.P. et al. (2006) Trends Neurosci 29, 452-8. 2 . Paul, S. et al. (2000) J Neurosci 20, 5630-8. 3 . Paul, S. et al. (2003) Nat Neurosci 6, 34-42. 4 . Braithwaite, S.P. et al. (2006) Eur J Neurosci 23, 2847-56. 5 . Zhang, Y. et al. (2008) J Neurosci 28, 10561-6. |
Western blot analysis of extracts from mouse and rat brain using STEP Antibody. Western blot分析小鼠和大鼠大脑提取物，使用的抗体是STEP Antibody 兔多抗。 |
