| 货号 | 12009S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | SSRP1 Antibody recognizes endogenous levels of total SSRP1 protein. This antibody also cross-reacts with a protein of unknown origin at 150 kDa. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Supressor of Ty-16 (SPT16) and structure-specific recognition protein-1 (SSRP1) are subunits of the facilitates chromatin transcription (FACT) complex that is essential for transcription elongation (1,2). FACT facilitates RNA polymerase-dependent transcription of chromatin templates by destabilizing the nucleosomes within the open reading frames of active genes (3-5). FACT destabilizes the nucleosomes, which would otherwise act as barriers to RNA polymerase transcription activity, by disrupting histone-histone and histone-DNA contacts that lead to the eviction of the histone H2A-H2B dimer (2,3,6). FACT may also function as a histone chaperone to reassemble nucleosomes after RNA polymerase passage (7). In addition to transcription, FACT activity has been shown to have a role in DNA replication in yeast and in DNA repair by contributing to the activation of p53 by CK2 and by facilitating histone H2AX-H2B exchange upon DNA damage (8-10). |
| 存放说明 | -20C |
| 计算分子量 | 81 |
| 参考文献 | 1 . Winkler, D.D. and Luger, K. (2011) J Biol Chem 286, 18369-74. 2 . Orphanides, G. et al. (1999) Nature 400, 284-8. 3 . Orphanides, G. et al. (1998) Cell 92, 105-16. 4 . Birch, J.L. et al. (2009) EMBO J 28, 854-65. 5 . Orphanides, G. and Reinberg, D. (2000) Nature 407, 471-5. 6 . Keller, D.M. and Lu, H. (2002) J Biol Chem 277, 50206-13. 7 . Belotserkovskaya, R. et al. (2003) Science 301, 1090-3. 8 . Schlesinger, M.B. and Formosa, T. (2000) Genetics 155, 1593-606. 9 . Keller, D.M. and Lu, H. (2002) J Biol Chem 277, 50206-13. 10 . Heo, K. et al. (2008) Mol Cell 30, 86-97. |
Western blot analysis of extracts from various cell lines using SSRP1 Antibody.Western blot方法检测不同细胞系的提取物，使用的抗体为 SSRP1 Antibody。 |
