| 货号 | 8162S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Sec23A Antibody recognizes endogenous levels of total Sec23A protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | There are two isoforms of Sec23 protein: Sec23A and Sec23B. Both isoforms have been shown in the Sec23/24 complex, which is a component of COPII coat (1). COPII is composed of at least five proteins: the Sec23/24 complex, the Sec13/31 complex, and Sar1. COPII coat is located at the ER/Golgi interface and involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (2). COPII formation is initiated through binding of the activated G protein, Sar1, to the Sec23/24 complex to form a prebudding complex, which directly binds target molecules (2-4). The prebudding complex further recruits Sec13/31 to form mature COPII coat (5,6). In addition to being a COPII component, Sec23 has also been shown to interact with p125 and Sec16 at the transitional ER; these interactions are important for regulation of the COPII transportation function (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 82 |
| 参考文献 | 1 . Paccaud, J.P. et al. (1996) Mol Biol Cell 7, 1535-46. 2 . Aridor, M. et al. (1998) J Cell Biol 141, 61-70. 3 . Miller, E.A. et al. (2003) Cell 114, 497-509. 4 . Mossessova, E. et al. (2003) Cell 114, 483-95. 5 . Barlowe, C. et al. (1994) Cell 77, 895-907. 6 . Bi, X. et al. (2007) Dev Cell 13, 635-45. 7 . Iinuma, T. et al. (2007) J Biol Chem 282, 17632-9. 8 . Hughes, H. et al. (2009) J Cell Sci 122, 2924-34. |
Western blot analysis of extracts from various cell lines using Sec23A Antibody. 使用Sec23A Antibody，免疫印迹（Western Blot）分析不同细胞中Sec23A蛋白水平。 |
