| 货号 | 9333S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | RSK1 Antibody detects endogenous levels of RSK1. It does not cross-react with the RSK2 or RSK3 isoforms. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
| 参考文献 | 1 . Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9. 2 . Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8. 3 . Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505. 4 . Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45. 5 . Um, S.H. et al. (2006) Cell Metab 3, 393-402. 6 . Chaturvedi, D. et al. (2006) Mol Cell Biol 26, 4586-600. 7 . Ruvinsky, I. et al. (2005) Genes Dev 19, 2199-211. |
Western blot analysis of purified RSK1, RSK2, and RSK3 proteins using RSK1 Antibody (upper), RSK2 Antibody #9340 (middle) or RSK3 Antibody #9343 (lower).Western blot方法检测：纯化的RSK1, RSK2,RSK3蛋白。使用的抗体是RSK1 Antibody (上图), RSK2 Antibody #9340 (中图)和RSK3 Antibody #9343 (下图)。 | |
Western blot analysis of extracts from HeLa and COS cells using RSK1 Antibody.Western blot方法检测细胞提取物：HeLa、 COS细胞。使用的抗体是RSK1 Antibody。 |
