| 货号 | 5844S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | RMP Antibody recognizes endogenous levels of total RMP protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | RMP (RPB5-Mediating Protein), also known as URI (Unconventional prefoldin RBP5 Interactor), was described as an unconventional member of the prefoldin (PFD) family of chaperones that are involved in actin and tubulin folding (1-4). Like conventional members of the α-class of PFDs, RMP contains N- and C-terminal α-helical coiled-coil structures connected by two β hairpins. In addition, RMP possesses an RPB5-binding segment and a long C-terminal acidic segment. It is posited that RMP exists as a component of a macromolecular complex within human cells and functions as a molecular scaffold to assemble a PFD complex containing other PFDs and proteins with functions in transcription and ubiquitination. Indeed, evidence is provided that RMP negatively modulates RNA polymerase II-dependent transcription by binding to TFIIF (5) and RBP5 (6) and is involved in mTOR signaling by coordinating the regulation of nutrient availability with gene expression (1). In accord with its ability to coordinate gene expression with nutrient availability, RMP was shown to be a mitochondrial substrate of S6K1. S6K1-mediated phosphorylation of RMP at Ser371 triggers a series of biochemical events that constitute a negative feedback loop, in part, aimed at restraining S6K1 survival signaling and ensuring that the mitochondrial threshold for apoptosis corresponds to availability of nutrients and growth factors (7).RMP (RPB5介导的蛋白)又称为URI (Unconventional prefoldin RBP5 Interactor)，其被描述作为伴侣蛋白prefoldin (PFD)家族的一个非传统成员，其涉及actin和tubulin蛋白的折叠 (1-4)。像PFDs的α类传统成员一样，RMP蛋白含有通过两个β发夹结构连接的N和C端的α-螺旋型卷曲螺旋结构。此外，RMP蛋白拥有一个RPB5蛋白结合部分和一个长的羧基末端酸性部分。研究指出RMP作为一个人类细胞大分子复合物的组成成分，且作为一个分子支架来组装PFD复合物，这个复合物包含其它PFDs 和转录及泛素化功能相关的蛋白。的确，研究证明RMP通过结合到TFIIF (5)和RBP5 (6)负调控RNA polymerase II依赖的转录，并且通过协调与基因表达有关的营养成分的供给从而涉及mTOR 信号通路(1)。与营养成分供给相关的基因表达的协调能力相符合，RMP被证实是一个S6K1的线粒体底物。S6K1介导RMP在Ser371 位点的磷酸化可引起一系列生物化学反应，反应可构成一个负反馈环，某种程度上，针对抑制S6K1存活信号和确保线粒体凋亡的阈值应与营养和生长因子的供给相符合(7)。 |
| 存放说明 | -20C |
| 计算分子量 | 79 |
| 参考文献 | 1 . Gstaiger, M. et al. (2003) Science 302, 1208-12. 2 . Vainberg, I.E. et al. (1998) Cell 93, 863-73. 3 . Martín-Benito, J. et al. (2002) EMBO J 21, 6377-86. 4 . Geissler, S. et al. (1998) EMBO J 17, 952-66. 5 . Wei, W. et al. (2003) Cell Res 13, 111-20. 6 . Dorjsuren, D. et al. (1998) Mol Cell Biol 18, 7546-55. 7 . Djouder, N. et al. (2007) Mol Cell 28, 28-40. |
Western blot analysis of extracts from various cell lines using RMP Antibody. 使用RMP Antibody，免疫印迹（Western Blot）分析在不同细胞系中RMP蛋白水平。 | |
Western blot analysis of extracts from COS-7 cells, either mock transfected (-) or transfected with human RMP encoding transcript variant 1 (+), using RMP Antibody. 使用RMP Antibody，免疫印迹（Western Blot）分析在COS-7细胞系中RMP的蛋白水平，细胞分别用空白对照 (-)和编码转录本变构体1的人类RMP转染(+)。 |
