| 货号 | 5754S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | RCHY1 Antibody detects endogenous levels of total RCHY1 protein. This antibody does not detect RCHY1 dimers. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | RCHY1(RING finger and CHY1 zinc finger domain-containing protein 1)是新发现的RING-H2型蛋白泛素化E3连接酶，由于其mRNA存在可变剪接而有多个蛋白亚型(1,2)。值得注意的是， RCHY1是p53的调节基因，有多项研究显示RCHY1与p53直接结合并促使其通过泛素化依赖的蛋白酶体系统降解，最终导致p53介导的生长抑制性转录过程减弱(1,3)。RCHY1也通过促进CDK抑制分子如p27的降解，与细胞增殖失调有关(4)。与其生长促进作用相一致，RCHY1过表达与肺癌(5,6)和前列腺癌(7)发病有关。 |
| 存放说明 | -20C |
| 计算分子量 | 29 |
| 参考文献 | 1 . Corcoran, C.A. et al. (2009) J Biol Chem 284, 21955-70. 2 . Shi, J. et al. (2010) Mol Cell Pharmacol 2, 21-23. 3 . Leng, R.P. et al. (2003) Cell 112, 779-91. 4 . Hattori, T. et al. (2007) Cancer Res 67, 10789-95. 5 . Duan, W. et al. (2004) J Natl Cancer Inst 96, 1718-21. 6 . Duan, W. et al. (2006) Exp Cell Res 312, 3370-8. 7 . Logan, I.R. et al. (2006) Mol Cell Biol 26, 6502-10. |
Western blot analysis of extracts from various cell lines using RCHY1 Antibody. | |
Western blot analysis of extracts from 293T cells, either mock transfected or transfected with a construct overexpressing murine RCHY1, using RCHY1 Antibody. |
