| 货号 | 3330S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | PTPA/PPP2R4 Antibody detects endogenous levels of total PTPA protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). Active protein phosphatase 2A is composed of both structural (A) and catalytic (C) proteins, and its activity relies on interaction with regulatory (B) subunits. An important PP2A regulatory subunit is PP2A phosphatase activator (PTPA), also known as the PP2A activator regulatory subunit 4 (PPP2R4) (4). This PTPA regulatory protein binds ATP and has isomerase (PPIase) activity, suggesting that PP2A regulation involves a change in phosphatase conformation. The addition of ATP (and Mg2+) results in a correlated increase in both PP2A activation and PTPA isomerase activity (5). While the exact mechanism is still under consideration, evidence suggests that binding of PTPA to the PP2A A-C dimer produces a conformational change in PP2A that shifts phosphatase substrate specificity from phosphoserine to phosphotyrosine substrates (6).蛋白磷酸酶2A (PP2A)是一种重要的蛋白丝氨酸/苏氨酸磷酸酶，在所有的真核细胞中结构保守。PP2A在各种细胞信号转导通路中都是关键的酶，它调节基本的细胞活动如DNA复制、转录、翻译、代谢、细胞周期进程、细胞分化、细胞凋亡和发育(1-3)。活性蛋白磷酸酶2A是由结构亚基A和催化亚基C组成，其活性依赖于和B亚基的相互作用。一个重要的PP2A调节亚基是PP2A磷酸酶活化剂(PTPA)，亦被称为PP2A活化剂调节亚基4(PPP2R4) (4)。该PTPA调节蛋白结合ATP且具有异构酶(PPIase)活性，表明PP2A调节涉及磷酸酶的构象改变。ATP和Mg2+的增加导致相关的PP2A激活增加和PTPA异构酶活性增加(5)。尽管具体的机制尚在研究中，有证据表明PTPA结合到PP2A A-C二聚体能够导致PP2A的构象变化，改变磷酸酶底物特异性从磷酸丝氨酸到磷酸酪氨酸底物(6)。 |
| 存放说明 | -20C |
| 计算分子量 | 40 |
| 参考文献 | 1 . Janssens, V. and Goris, J. (2001) Biochem J 353, 417-39. 2 . Zolnierowicz, S. (2000) Biochem Pharmacol 60, 1225-35. 3 . Millward, T.A. et al. (1999) Trends Biochem Sci 24, 186-91. 4 . Fellner, T. et al. (2003) Genes Dev 17, 2138-50. 5 . Jordens, J. et al. (2006) J Biol Chem 281, 6349-57. 6 . Chao, Y. et al. (2006) Mol Cell 23, 535-46. |
Western blot analysis of extracts from various cell lines using PTPA/PPP2R4 Antibody.Western blot方法检测不同细胞提取物，使用的抗体为PTPA/PPP2R4 Antibody. |
