| 货号 | 9552S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PTEN Antibody detects endogenous levels of total PTEN protein. The antibody does not cross-react with related proteins. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10). |
| 存放说明 | -20C |
| 计算分子量 | 54 |
| 参考文献 | 1 . Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5. 2 . Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7. 3 . Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8. 4 . Wan X and Helman LJ (2003) Oncogene 22, 8205–11 5 . Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91. 6 . Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8. 7 . Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8. 8 . Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30. 9 . Funamoto, S. et al. (2002) Cell 109, 611-23. 10 . Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610. |
Western blot analysis of extracts from various cell lines, using PTEN Antibody. Western blot 分析不同细胞系的提取物，所用抗体为PTEN Antibody 。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® PTEN siRNA I (+), using PTEN Antibody #9552 and p42 MAPK (Erk2) Antibody #9108. PTEN Antibody confirms silencing of PTEN expression, while the p42 MAPK (Erk2) Antibody is used to control for loading and specificity of PTEN siRNA. Western blot 分析转染了100nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) 或者SignalSilence® PTEN siRNA I (+)的HeLa 细胞提取物，使用的抗体为 PTEN Antibody #9552 和p42 MAPK (Erk2) Antibody #9108。PTEN Antibody 证实了PTEN表达的缺失，而p42 MAPK (Erk2) Antibody是作为PTEN siRNA上样与特异性的对照。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® PTEN siRNA II (+), using PTEN (138G6) Rabbit mAb and β-Actin (13E5) Rabbit mAb #4970. PTEN (138G6) Rabbit mAb confirms silencing of PTEN expression, while the β-Actin (13E5) Rabbit mAb is used to control for loading and specificity of PTEN siRNA。 Western blot 分析转染了100nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) 或者SignalSilence® PTEN siRNA I (+)的HeLa 细胞提取物，使用的抗体为 PTEN (138G6) Rabbit mAb 和 β-Actin (13E5) Rabbit mAb兔单抗 #4970。PTEN (138G6) Rabbit mAb 兔单抗证实了PTEN表达的缺失，而β-Actin (13E5) Rabbit mAb 兔单抗是作为PTEN siRNA上样与特异性的对照。 |
