| 货号 | 2464S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PTCH2 (L849) Antibody detects transfected levels of PTCH2 protein. It does not recognize transfected levels of human PTCH1. This antibody also cross-reacts with an unidentified protein at 55 kDa. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Transfected Only |
| 背景 | Patched1 and 2 (PTCH1 and PTCH2) are twelve-pass transmembrane proteins that function as the receiving receptors for members of the Hedgehog family of proteins (1-4). In the absence of Hedgehog proteins, PTCH suppresses the otherwise constitutively active signaling receptor Smoothened (Smo) so that the Hedgehog signaling pathway is in the off state (5,6). Deactivating mutations that impair the ability of PTCH1 to suppress Smo are frequently found in patients with nevoid basal cell carcinoma syndrome (7,8). PTCH proteins have a sterol-sensing domain (SSD) also found in several proteins that function in cholesterol homeostasis, such as HMGCR (3-hydroxy-3-methylglutaryl coenzyme A-reductase) and SCAP (sterol regulatory element-binding protein-cleavage activating protein). However, the role of the SSD in Patched proteins is not clear (9,10). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
| 参考文献 | 1 . Stone, D.M. et al. (1996) Nature 384, 129-34. 2 . Chen, Y. and Struhl, G. (1996) Cell 87, 553-63. 3 . Motoyama, J. et al. (1998) Nat Genet 18, 104-6. 4 . Smyth, I. et al. (1999) Hum Mol Genet 8, 291-7. 5 . Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059-87. 6 . McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1-114. 7 . Hahn, H. et al. (1996) Cell 85, 841-851. 8 . Johnson, R.L. et al. (1996) Science 272, 1668-1671. 9 . Kuwabara, P.E. and Labouesse, M. (2002) Trends Genet. 18, 193-201. 10 . Chang, T.Y. et al. (2006) Annu. Rev. Cell Dev. Biol. 22, 129-157. |
Western blot analysis of total cell lysates from COS cells and COS cells transiently transfected with a construct expressing human PTCH2 using PTCH2 (L849) Antibody. 使用PTCH2 (L849)抗体对COS细胞和瞬转表达人PTCH2的COS细胞的全细胞提取物进行western blot分析。 |
