| 货号 | 13378S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | PSMG1/PAC1 Antibody recognizes endogenous levels of total PSMG1 (PAC1) protein in human and monkey cells. This antibody weakly cross-reacts with murine and rat PSMG1 (PAC1) protein. This antibody does not cross-react with PSMG2 (PAC2) protein. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Proteasome assembly chaperone 1 (PSMG1, PAC1) is an evolutionarily conserved, ubiquitously expressed chaperone protein that promotes proper biogenesis of the α-ring of the 20S CP of the eukaryotic proteasome (3,4). PSMG1 (PAC1) functions in a heterodimeric complex with PSMG2 (PAC2) and was originally identified as a proteasome subunit binding partner (4). Intact PSMG1-PSMG2 heterodimers both promote heteroheptameric α-ring assembly and/or stability and prevent accumulation of non-productive α-ring dimers (4). Research studies targeting the disruption of the murine Psmg1 locus have substantiated the importance of proteasome chaperones in contributing to normal proteasome maturation and cellular homeostasis by demonstrating that loss of Psmg1/Pac1 function leads to embryonic lethality (5).蛋白酶体组装伴侣蛋白1(PSMG1 PAC1)是一个进化保守,广泛表达的伴侣蛋白,促进真核蛋白酶体20S CP的α环状的特有生物合成(3、4)。PSMG1(PAC1)与PSMG2 (PAC2)形成异源复合体发挥作用，并最早被鉴定为蛋白酶体亚基结合蛋白(4)。完整PSMG1-PSMG2异源二聚体既促进异源二聚体α环装配和/或稳蛋白定性，并阻止非产α环二聚体的积累(4)。研究表明小鼠Psmg1基因位点的缺失已经证实蛋白酶体伴侣的重要性，其与正常的蛋白酶体成熟和细胞内稳态息息相关。实验已证明缺失Psmg1 / Pac1将会导致胚胎致死(5)。 |
| 存放说明 | -20C |
| 计算分子量 | 30 |
| 参考文献 | 1 . Finley, D. (2009) Annu Rev Biochem 78, 477-513. 2 . Lee, M.J. et al. (2011) Mol Cell Proteomics 10, R110.003871. 3 . Vidal-Taboada, J.M. et al. (2000) Biochem Biophys Res Commun 272, 156-63. 4 . Hirano, Y. et al. (2005) Nature 437, 1381-5. 5 . Sasaki, K. et al. (2010) Mol Cell Biol 30, 3864-74. |
Western blot analysis of extracts from various cell lines using PSMG1/PAC1 Antibody.Western blot检测多种细胞提取物，使用的抗体是PSMG1/PAC1 Antibody. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human PSMG1 protein (hPSMG1-Myc/DDK; +) or Myc/DDK-tagged full-length human PSMG2 protein (hPSMG2-Myc/DDK; +), using PSMG1/PAC1 Antibody (upper) and DYKDDDDK Tag Antibody #2368 (lower).Western blot分析293T细胞提取物，对照未转染（-）或转染了Myc/DDK标签的全长人源的PSMG1 protein (hPSMG1-Myc/DDK; +)，或Myc/DDK标签的全长人源的PSMG2 protein (hPSMG2-Myc/DDK; +)，使用的抗体是PSMG1/PAC1 Antibody (上图) and DYKDDDDK Tag Antibody #2368 (下图)。 | |
Immunoprecipitation of PSMG1 from 293T cell extracts using Normal Rabbit IgG #2729 (lane 2) or PSMG1/PAC1 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PSMG1/PAC1 Antibody.对293T细胞提取物中的PSMG1进行免疫共沉淀，使用抗体是Normal Rabbit IgG #2729 (泳道2)或PSMG1/PAC1 Antibody(泳道3)。泳道1为10%的上样。Western blot分析使用抗体是PSMG1/PAC1 Antibody。 |
