| 货号 | 13392S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | PSMC5/TRIP1 Antibody recognizes endogenous levels of total PSMC5 (TRIP1) protein. This antibody does not cross-react with other AAA-ATPase subunits of the 19S proteasome regulatory particle. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The eukaryotic proteasome 19S RP contains six ATPases-associated-with-different-cellular-activities (AAA) proteins (PSMC1-PSMC6) that bind directly to the 20S CP α-ring. These 19S RP ATPases are thought to assemble into a heterohexameric, pore-like structure that forms part of the substrate translocation channel. Energy derived from ATP hydrolysis by the AAA-ATPases is utilized for substrate unfolding and translocation, which is required for degradation of ubiquitinated folded proteins within the central chamber of the 20S CP formed by β-subunits (3-5). Thyroid hormone receptor-interacting protein 1 (PSMC5, TRIP1) is a 19S AAA-ATPase subunit involved in the negative regulation of gene transcription. Recruitment of PSMC5 to liganded VDR (6) and RARγ2 (7) facilitates their degradation via the proteasome. |
| 存放说明 | -20C |
| 计算分子量 | 45 |
| 参考文献 | 1 . Finley, D. (2009) Annu Rev Biochem 78, 477-513. 2 . Lee, M.J. et al. (2011) Mol Cell Proteomics 10, R110.003871. 3 . Groll, M. et al. (2000) Nat Struct Biol 7, 1062-7. 4 . Braun, B.C. et al. (1999) Nat Cell Biol 1, 221-6. 5 . Liu, C.W. et al. (2002) J Biol Chem 277, 26815-20. 6 . Masuyama, H. and MacDonald, P.N. (1998) J Cell Biochem 71, 429-40. 7 . Giannì, M. et al. (2002) EMBO J 21, 3760-9. |
Western blot analysis of extracts from various cell lines using PSMC5/TRIP1 Antibody.Western blot检测多种细胞提取物，使用的抗体是PSMC5/TRIP1 Antibody. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human PSMC1 (hPSMC1-Myc/DDK; +), full-length human PSMC2 (hPSMC2-Myc/DDK; +), full-length human PSMC3 (hPSMC3-Myc/DDK; +), full-length human PSMC4 (hPSMC4-Myc/DDK; +); full-length human PSMC5 (hPSMC5-Myc/DDK; +), or full-length human PSMC6 (hPSMC6-Myc/DDK; +), using PSMC5/TRIP1 Antibody (upper) and DYKDDDDK Tag Antibody #2368 (lower).Western blot分析293T细胞提取物，对照未转染（-）或转染了Myc/DDK标签的全长人源的PSMC1 (hPSMC1-Myc/DDK; +), Myc/DDK标签的全长人源的PSMC2 (hPSMC2-Myc/DDK; +)，Myc/DDK标签的全长人源的PSMC3 (hPSMC3-Myc/DDK; +)，Myc/DDK标签的全长人源的PSMC4 (hPSMC4-Myc/DDK; +)，Myc/DDK标签的全长人源的PSMC5 (hPSMC5-Myc/DDK; +)，或Myc/DDK标签的全长人源的PSMC6 (hPSMC6-Myc/DDK; +)，使用的抗体是PSMC5/TRIP1 Antibody (上图) and DYKDDDDK Tag Antibody #2368 (下图)。 |
