| 货号 | 13923S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | PSMC3/TBP1 recognizes endogenous levels of total PSMC3 (TBP1) protein. This antibody does not cross-react with other AAA-ATPase subunits of the 19S proteasome regulatory particle. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The base of the eukaryotic proteasome 19S/PA700 RP contains six AAA-ATPase subunits (PSMC1-PSMC6) that bind directly to the 20S CP α-ring. These 19S RP ATPases are thought to assemble into a heterohexameric, pore-like structure that forms part of the substrate translocation channel. Energy derived from ATP hydrolysis by the AAA-ATPases is utilized for substrate unfolding and translocation, which is required for degradation of ubiquitinated folded proteins within the central chamber of the 20S CP formed by β-subunits (3-5). The human immunodeficiency virus Tat-Binding Protein 1 (PSMC3, TBP1) is a 19S AAA-ATPase subunit that functions as a transcriptional activator (6-8). Research studies demonstrate that PSMC3/TBP1 may act as a tumor suppressor by promoting pVHL-dependent degradation of HIF1α (9) and protecting p14-ARF from proteasomal degradation (10). |
| 存放说明 | -20C |
| 计算分子量 | 46, 48 |
| 参考文献 | 1 . Finley, D. (2009) Annu Rev Biochem 78, 477-513. 2 . Lee, M.J. et al. (2011) Mol Cell Proteomics 10, R110.003871. 3 . Groll, M. et al. (2000) Nat Struct Biol 7, 1062-7. 4 . Braun, B.C. et al. (1999) Nat Cell Biol 1, 221-6. 5 . Liu, C.W. et al. (2002) J Biol Chem 277, 26815-20. 6 . Corn, P.G. et al. (2003) Nat Genet 35, 229-37. 7 . Nelbock, P. et al. (1990) Science 248, 1650-3. 8 . Ohana, B. et al. (1993) Proc Natl Acad Sci U S A 90, 138-42. 9 . Satoh, T. et al. (2009) Endocrinology 150, 3283-90. 10 . Pollice, A. et al. (2007) Oncogene 26, 5154-62. |
Western blot analysis of extracts from various cell lines using PSMC3/TBP1 Antibody. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human PSMC1 protein (hPSMC1-Myc/DDK; +), full-length human PSMC2 protein (hPSMC2-Myc/DDK; +), full-length human PSMC3 protein (hPSMC3-Myc/DDK; +), full-length human PSMC4 protein (hPSMC4-Myc/DDK; +), full-length human PSMC5 protein (hPSMC5-Myc/DDK; +), and full-length human PSMC6 protein (hPSMC6-Myc/DDK; +), using PSMC3/TBP1 Antibody (upper) and DYKDDDDK Tag Antibody #2368 (lower). |
