| 货号 | 3715S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Pro-TGF-alpha Antibody detects enogenous levels TGF-alpha precusor protein but not mature TGF-alpha. It does not cross-react with other family members at physiological levels. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Transforming growth factor alpha (TGF-alpha) is a member of the epidermal growth factor (EGF) family, sharing the same receptor, EGFR, and regulating cell proliferation, survival, and differention (1). Members of the family share an EGF-like domain of 45-60 amino acids characterized by the conservation of six regularly spaced cysteines, forming three disulfide bonds that function as their receptor binding domain. TGF-alpha was initially discovered in the media of retrovirally transformed fibroblasts, and it name comes from its ability to induce transformation in cultured fibroblasts (2). This transforming activity was later shown to require TGF-beta, which potentiates the activity of TGF-alpha through a separate receptor (3). Soluble TGF-alpha is released from its membrane-bound precusor, pro-TGF-alpha, following protolytic cleavage, but the membrane bound precursor is still able to bind and activate EGFR (4). Binding of soluble or membrane bound TGF-alpha to EGFR leads to receptor dimerization, tyrosine autophosphorylation, and activation of downstream signaling components. TGF-alpha and related peptides play an important role in the progression of cancer as well as in neuropathological processes (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 17 |
| 参考文献 | 1 . Derynck, R. (1986) J Cell Biochem 32, 293-304. 2 . de Larco, J.E. and Todaro, G.J. (1978) Proc Natl Acad Sci U S A 75, 4001-5. 3 . Roberts, A.B. et al. (1981) Proc Natl Acad Sci U S A 78, 5339-43. 4 . Wong, S.T. et al. (1989) Cell 56, 495-506. 5 . Rusch, V. et al. (1996) Cytokine Growth Factor Rev 7, 133-41. 6 . Junier, M.P. (2000) Prog Neurobiol 62, 443-73. |
Western blot analysis of extracts from THP-1, Jurkat, and Ramos cells, using Pro-TGF-alpha Antibody.使用Pro-TGF-alpha 抗体对THP-1, Jurkat和Ramos 细胞提取物进行western blot分析。 |
