| 货号 | 3639S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | PRC1 Antibody recognizes endogenous levels of total PRC1 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Protein Regulator of Cytokinesis 1 (PRC1) is a member of the MAP65/ASE1 family of nonmotor microtubule-associated proteins, first described in budding yeast (1,2). PRC1 is a substrate of CDK1, which maintains PRC1 in an inactive, monomeric state (2). Cell-cycle dependent degradation of CDK1 leads to dephosphorylation of PRC1 and subsequent KIF4-mediated translocation to the plus ends of microtubules, where it promotes microtubule bundling by cross-linking antiparallel microtubules (3-5). The microtubule bundling functions of PRC1 play a critical role in maintaining structural integritry of the spindle midzone during cytokinesis (3,6,7). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
| 参考文献 | 1 . Pellman, D. et al. (1995) J Cell Biol 130, 1373-85. 2 . Jiang, W. et al. (1998) Mol Cell 2, 877-85. 3 . Kurasawa, Y. et al. (2004) EMBO J 23, 3237-48. 4 . Zhu, C. and Jiang, W. (2005) Proc Natl Acad Sci USA 102, 343-8. 5 . Subramanian, R. et al. (2010) Cell 142, 433-43. 6 . Mollinari, C. et al. (2002) J Cell Biol 157, 1175-86. 7 . Zhu, C. et al. (2006) Proc Natl Acad Sci USA 103, 6196-201. |
Western blot analysis of extracts from various cell lines using PRC1 Antibody.使用PRC1抗体对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from HeLa cells, untreated (-) or nocodazole-treated (+; 100 ng/ml overnight) to induce mitotic arrest and increase CDK1 activity. Increased CDK1 activity induces PRC1 phosphorylation, as indicated by the upper band.未处理(-)或nocodazole处理(+; 100 ng/ml过夜)以诱导有丝分裂中止和增加CDK1活性的HeLa细胞提取物进行western blot分析。增加的CDK1活性会诱导PRC1磷酸化，正如上方条带指出。 |
