货号 | 3470S |
反应种属 | Human/Mouse |
来源宿主 | Rabbit |
应用 | W/IHC-P/IF-IC |
目标/特异性 | Perilipin (D418) Antibody detects endogenous levels of total perilipin protein. |
使用方法 | WB(1:1000) IHC-P (1:100) IF-IC (1:100) |
供应商 | CST |
背景 | Triacylglycerol is stored in lipid droplets as a primary energy reserve. Perilipin is localized at the periphery of lipid droplets and serves as a protective coating against lipases (1-3). Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin (1,2,4,5). Phosphorylation of perilipin results in the conformational change that exposes lipid droplets to endogenous lipases, such as hormone-sensitive lipases (2). Hence, perilipin plays a pivotal role in lipid storage (2,5). |
存放说明 | -20C |
计算分子量 | 62 |
Immunohistochemical analysis of paraffin-embedded mouse brown fat using Perilipin (D418) Antibody in the presence of control peptide (left) or antigen-specific peptide (right).免疫组织化学检测石蜡包埋的小鼠棕色脂肪组织,所用抗体为Perilipin (D418) Antibody。左为对照肽,右为抗原特异性肽。 | |
Immunohistochemical analysis of paraffin-embedded 3T3-L1 cells, control (left) or differentiated (right), using Perilipin (D418) Antibody.免疫组织化学检测石蜡包埋的3T3-L1 细胞,左为对照,右为分化的细胞,所用抗体为Perilipin (D418) Antibody。 | |
Confocal immunofluorescent analysis of differentiated 3T3-L1 cells using Perilipin (D418) Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光检测3T3-L1 细胞,所用抗体为Perilipin (D418) Antibody(绿),蓝色伪彩为DNA荧光染料(对应产品号为DRAQ5 #4084)。 | |
Western blot analysis of extracts from NIH/3T3 and differentiated 3T3-L1 cells using Perilipin (D418) Antibody.Wetern blot检测 NIH/3T3 和分化的 3T3-L1 细胞提取物,所用抗体为Perilipin (D418) Antibody。 |