| 货号 | 4342S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Pbx1 Antibody detects endogenous levels of total Pbx1a and Pbx1b proteins. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | The Pbxs (Pre-B cell leukemia transcription factors) belong to the Three Amino Acid Loop Extension (TALE) family of homeodomain containing protein (1). Pbx1 has two isoforms, Pbx1a and 1b, that are divergent in sequence at the carboxy terminus, which is a result of alternative mRNA splicing. Unlike other homeoselector genes that have restricted expression in development Pbx genes are ubiquitously expressed in both fetal and adult tissue (1). Human pre-B cell acute leukemias are frequently associated with a t(1;19)(q23;p13.3) translocation that results in a fusion between the E2A and Pbx1 genes (2,3). Pbx1 genes are not normally expressed in lymphoid tissues, and the E2A-Pbx1 fusion protein is a potent transcription factor that drives aberrant gene expression. |
| 存放说明 | -20C |
| 计算分子量 | 42 kDa, 52 |
Western blot analysis of extracts from A431, HeLa and HEL cells, using Pbx1 Antibody.Western免疫印迹。用Pbx1 Antibody研究A431, HeLa 和 HEL细胞的细胞提取液。 | |
Western blot analysis of extracts from E10.5 mouse Pbx1 heterozygote or null emrbryos. Image kindly provided by Dr. Sheldon Rowan, Division of Genetics, Brigham and Womens Hospital, Harvard Medical School.Western免疫印迹。研究E10.5小鼠的Pbx1杂合子或者阴性胚胎的提取液。此图由Dr. Sheldon Rowan, Division of Genetics, Brigham and Womens Hospital, Harvard Medical School提供。 | |
Confocal immunofluorescent analysis of HeLa cells using Pbx1 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).共聚焦免疫荧光分析HeLa细胞，所用抗体为Pbx1 Antibody (绿色)。肌动蛋白丝用 DY-554 phalloidin (红色)标记。 |
