| 货号 | 2145S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | NSF Antibody detects endogenous levels of total NSF protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the α-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 78 |
Western blot analysis of extracts from SHSY5Y, Neuro2A, PC12 and CHO cells using NSF Antibody. 对SHSY5Y, Neuro2A, PC12和CHO细胞裂解液使用NSF抗体进行Western blot分析。 | |
Immunoprecipitation of NSF from untreated HeLa cell extracts using NSF antibody (Lane 3). Lane 1: Input control. Lane 2: No antibody control. 对未处理的Hela细胞裂解液使用NSF antibody（第三列）进行免疫共沉淀分析。1泳道：输入对照。2泳道：无抗体对照。 |
