| 货号 | 5196S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | NDRG1 Antibody detects endogenous levels of total NDRG1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | N-myc downstream-regulated gene 1 (NDRG1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG family, which is composed of four members (NDRG1-4) that function in growth, differentiation, and cell survival (1-5). NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage (4), hypoxia (5), and elevated levels of nickel and calcium (2). Expression of NDRG1 is elevated in N-myc defective mice and is negatively regulated by N- and c-myc (1,6). During DNA damage, NDRG1 is induced in a p53-dependent fashion and is necessary for p53-mediated apoptosis (4,7). Research studies have shown that NDRG1 may also play a role in cancer progression by promoting differentiation, inhibiting growth, and modulating metastasis and angiogenesis (3,4,6,8,9). Nonsense mutation of the NDRG1 gene has been shown to cause hereditary motor and sensory neuropathy-Lom (HMSNL), which is supported by studies demonstrating the role of NDRG1 in maintaining myelin sheaths and axonal survival (10,11). NDRG1 is up-regulated during mast cell maturation and its deletion leads to attenuated allergic responses (12). Both NDRG1 and NDRG2 are substrates of SGK1, although the precise physiological role of SGK1-mediated phosphorylation is not known (13). NDRG1 is phosphorylated by SGK1 at Thr328, Ser330, Thr346, Thr356, and Thr366. Phosphorylation by SGK1 primes NDRG1 for phosphorylation by GSK-3. |
| 存放说明 | -20C |
| 计算分子量 | 46, 48 |
Western blot analysis of extracts from various cell lines using NDRG1 Antibody. 使用NDRG1抗体对多种细胞提取物进行Western blot分析。 | |
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with SignalSilence® NDRG1 siRNA II #6257, using NDRG1 Antibody (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower). 未转染或使用SignalSilence® NDRG1 siRNA II #6257转染后的HeLa细胞提取物Western blot分析，使用NDRG1抗体（上）或β-Tubulin (9F3)兔源单克隆抗体#2128（下）进行检测。 | |
Immunohistochemical analysis on SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101 [paraffin-embedded LNCaP cell pellets, control (left) or LY294002-treated (right)] using NDRG1 Antibody (top) or Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb #5482 (bottom). 使用NDRG1抗体（上）或Phospho-NDRG1 (Thr346) (D98G11) XP®兔单克隆抗体#5482（下）对SignalSlide® Phospho-Akt (Ser473)免疫组化对照#8101[石蜡包埋LNCaP细胞，对照组（左）或LY294002处理组（右）]进行免疫组化实验。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using NDRG1 Antibody. 使用NDRG1抗体对石蜡包埋的人结肠癌组织进行免疫组化。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using NDRG1 Antibody. 使用NDRG1对石蜡包埋的人肺癌组织进行免疫组化分析。 |
