| 货号 | 14760S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 背景 | The megakaryoblastic leukemia proteins 1 and 2 (MKL1, MKL2) are myocardin-related transcription factors (MRTF-A, MRTF-B) that serve as actin-regulated transcription coactivators for the serum response factor (SRF). Interaction between G-actin and MKL proteins retains the coactivator within the cytoplasm of resting cells. Activated Rho-A promotes F-actin assembly and a reduction of the G-actin pool in serum-stimulated cells. This results in the accumulation of MKL proteins in the nucleus, where the coactivator associates with the SRF to activate target gene transcription and mediate multiple cellular processes (1-4). A number of other signaling pathways, including the TGFβ, BMP, and PDGF pathways, also make use of MKL-mediated activation of target gene transcription (5-9). Chromosomal translocations involving the genes encoding MKL1 and MKL2 have been identified in several cases of acute megakaryoblastic leukemia and chondroid lipoma (10-12). |
| 存放说明 | -20C |
| 计算分子量 | 145 |
Western blot analysis of extracts from 293 and HeLa cells using MKL1/MRTF-A Antibody | |
Immunoprecipitation of MKL1/MRTF-A protein from 293 cell extracts using Normal Rabbit IgG #2729 (lane 2) or MKL1/MRTF-A Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using MKL1/MRTF-A Antibody. |
