| 货号 | 4472S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | HPK1 Antibody detects endogenous levels of total HPK1. The antibody does not cross-react with other members of the GCK family. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Hematopoietic progenitor kinase 1(HPK1) is a member of the germinal center kinases that is predominantly expressed in hematopoietic cells. HPK1 is a key component linking T or B cell receptors to SAPK/JNK and IkappaB kinase pathways in lymphocytes (1-3). Through its proline-rich motif, HPK1 associates with multiple SH3 domain-containing adaptor proteins, including GRB2, Nck, Crk, SLP-76, and BLNK. Phosphorylation of Tyr379 by Syk is necessary for HPK1 association with BLNK and SLP-76, as well as for HPK1 activity (4,5). Caspase cleavage of HPK1 also modulates the the biological function of HPK1 (3,6,7). |
| 存放说明 | -20C |
| 计算分子量 | 97 |
Western blot analysis of extracts from Jurkat (1), U-937 (2), THP1 (3), Raw 264.7 (4), WEHI 231 (5), and Ramos cells (6), using HPK1 Antibody.Western免疫印迹。用HPK1 Antibody研究Jurkat (1), U-937 (2), THP1 (3), Raw 264.7 (4), WEHI 231 (5) 和 Ramos cells (6)细胞的细胞提取液。 | |
Western blot analysis of extracts from WEHI231 cells, untreated or treated with staurosporine to induce HPK1 cleavage, using HPK1 Antibody.Western免疫印迹。用HPK1 Antibody研究未经处理的和经十字孢碱诱导HPK1断裂的WEHI231细胞的细胞提取液。 |
