| 货号 | 83393S |
| 反应种属 | Human\Moneky |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | UBE2O Antibody recognizes endogenous levels of total UBE2O protein. |
| 使用方法 | W |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1). |
| 存放说明 | -20C |
| 计算分子量 | 200 |
| 参考文献 | 1 . Hershko, A. (1988) J Biol Chem 263, 15237-40. 2 . Berleth, E.S. and Pickart, C.M. (1996) Biochemistry 35, 1664-71. 3 . Wefes, I. et al. (1995) Proc Natl Acad Sci U S A 92, 4982-6. 4 . Zhang, X. et al. (2013) EMBO J 32, 996-1007. |
Western blot analysis of extracts from various cell lines using UBE2O Antibody. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human UBE2O protein (hUBE2O-Myc/DDK; +), using UBE2O Antibody (upper) and DYKDDDDK Tag Antibody #2368 (lower). |
