| 货号 | 4914S |
| 反应种属 | Human/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Hic-5 Antibody detects endogenous levels of total Hic-5/ARA55 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Hic-5 is a LIM domain family member orginally identified as a TGFbeta1 and hydrogen peroxide inducible gene, and is nearly identical to the androgen receptor co-activator ARA55 (1-3). Hic-5 is structurally related to paxillin, and both proteins are localized to focal adhesions and thought to serve as adaptor molecules, linking signals from the extracellular matrix to cytoskeletal regulation and intracelluar signaling (4,5). Like paxillin, Hic-5 contains four LD motifs and four LIM domains. Expression of Hic-5 can affect cell growth and differentiation (6-8). Increased expression of Hic-5 is observed during cellular senescence in fibroblasts, and ectopic expression in immortalized fibroblasts suppressed cell growth (8). Unlike paxillin, Hic-5 may translocate to the nucleus in response to oxidants like hydrogen peroxide (9). It has been proposed that Hic-5 serves to shuttle redox signaling from focal adhesions to the nucleus where it acts as a transcriptional co-activator for some transciption factors including, Sp1 and PPARgamma (7,9,10). Phosphorylation of Hic-5 at Tyr60 by CAKbeta and Fyn may activiate Hic-5 signaling by allowing binding to downstream SH2 domain containing proteins (11). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
Western blot analysis of extracts from U-2-OS, COS-7 and A-431 cells using Hic-5 Antibody.使用Hic-5抗体对U-2-OS，COS-7和A-431细胞提取物进行western blot分析。 | |
Immunoprecipitation of Hic-5 from 293 cell lysates using Hic-5 Antibody or rabbit IgG control. Western blot was performed using a Hic-5 Mouse mAb.293细胞提取物使用Hic-5抗体或对照兔源IgG对Hic-5进行免疫沉淀实验。使用Hic-5鼠单抗进行western blot分析。 |
