| 货号 | 3992S |
| 反应种属 | Human/Mouse/Rat/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Gα (pan) Antibody detects total levels of endogenous G protein alpha subunits. The antibody may not detect G(12)-α, G(13)-α (13), G(s)-α (s) and G(olf)-α. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP3 formation (3). |
| 存放说明 | -20C |
| 计算分子量 | 39, 41 |
Western blot analysis of extracts from BAEC, HEK293, NIH/3T3, C6 and COS cells, using Gα (pan) Antibody. 对BAEC、HEK293、NIH/3T3、C6和COS细胞裂解物使用Gα(pan)抗体进行Western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Gα (pan) Antibody. 对石蜡包埋的人前列腺癌使用Gα(pan)抗体进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Gα (pan) Antibody in the presence of control peptide (left) or antigen specific peptide (right). 对石蜡包埋的人结肠癌使用Gα(pan)抗体进行免疫组织化学分析，其中左图为对照肽，右图为抗原特异肽。 |
